BEVERLY, Mass.--(BUSINESS WIRE)--Jun 26, 2024--
seqWell, a global provider of genomic library and multiplexing workflow solutions, today announced the launch of their new portfolio of Tagify loaded-transposase reagents, including Tagify i5 UMI adapter-loaded Tn5. In addition, seqWell will offer loading of transposases with customer-specified oligonucleotides, including loading of their high-performance TnX transposase.
This press release features multimedia. View the full release here: https://www.businesswire.com/news/home/20240626702033/en/
The Tagify portfolio provides a commercial source of fully quality-controlled, loaded transposases with a defined level of enzyme activity and payload verification. This eliminates the need of laboratories to purify, load, and characterize the final loaded transposase prior to use in their assays, freeing up laboratory resources and reducing a source of assay-to-assay variability.
“Assays to define the genome-wide on- and off-target activity of genome editors are important for both biomedical research and therapeutics,” said Shengdar Tsai, Ph.D, Associate Member, Department of Hematology, St. Jude Children’s Research Hospital. “Broadly adopted, sensitive, unbiased, biochemical and cellular methods of understanding the genome-wide activity of editors, such as CHANGE-seq and GUIDE-seq-2, rely on transposase-based tools. The commercial availability of standardized transposase reagents will help lower barriers for more labs to implement these assays rapidly and reliably.”
Tagify i5 UMI adapter-loaded transposase reagents support gene editing analysis assays, such as the UdiTaS™ method, that rely on tagmentation to simultaneously fragment and tag genomic DNA with Illumina-compatible adapters using a highly scalable workflow. These off-the-shelf reagents are available as a set of either 24 or 96 transposases each containing an i5 barcode and a unique molecular indentifier (UMI) for high throughput applications.
The Tagify custom-loading option enables scientists to address the growing number of NGS-based on- and off-target gene editing methods that rely on tagmentation via the flexibility to load either hyperactive Tn5 or seqWell’s recently introduced high performance TnX transposase, with Illumina-compatible barcodes, UMIs, universal adaptors, or a custom oligonucleotide sequence.
“Our early access customers valued the availability of a reliable source of quality transposase-based reagents with their payload of choice, and Tagify products build on seqWell’s expertise in transposase loading and manufacturing to deliver these tools” said Joe Mellor, Chief Scientific Officer at seqWell. “Tagify i5 UMI is a great example of this capability, focused on scientists developing new genome editing methods and therapeutics. The high level of batch-to-batch consistency and availability of up to 96 UMIs will support the reproducible and scalable analysis of on- and off-target effects in sequencing-based genome editing QC assays.”
For more information about the use of Tagify loaded transposase reagents, view an on-demand webinar entitled, “ Assessing CRISPR On-Target Editing and Structural Changes with UDiTaS™ using Tagify™ Reagents ” presented by Georgia Giannoukos, Ph.D., Director of Next Generation Sequencing, at Editas Medicine.
About seqWell
seqWell creates scalable genomics technologies that simplify workflows and help enable every life scientist to unlock transformative discoveries with sequencing. seqWell offers a range of next-generation library prep products and workflows that allow customers to achieve higher levels of sample multiplexing quickly and easily. Learn more about us at www.seqwell.com or follow us on Linkedin.
Tagify Adapter-loaded Transposase Reagents provide for reliable and scalable NGS-based on- and off-target gene editing analysis methods. (Photo: Business Wire)
NEW YORK (AP) — Paul Skenes pitched two perfect innings in his Yankee Stadium debut and the Pittsburgh Pirates hit five homers, including four off rookie Luis Gil, in a 9-4 win Saturday,
New York (93-68) later clinched home-field advantage throughout the American League playoffs when Cleveland (92-69) lost to Houston. The Yankees won the season series against the Guardians and hold the tiebreaker.
Yasmani Grandal and Nick Gonzales hit solo homers in the first four innings off Gil (15-7). Rookie Billy Cook in the fifth and Jared Triolo in the sixth added two-run drives. Nick Yorke hit a two-run homer in the ninth against Will Warren.
New York has lost four of five and opens the AL Division Series at home Oct. 5.
Aaron Judge followed his third game off this season with his third five-strikeout game, his first since May 5, 2021.
In front of 46,069, New York’s 17th home sellout, Skenes threw 23 pitches to six hitters. He threw four over 100 mph, including three to Juan Soto and Aaron Judge.
“He was outstanding,” Pirates manager Derek Shelton said. “We knew it was going to be a shorter start. This is a really tough lineup and he executed pitches throughout the first six hitters.”
With girlfriend and LSU gymnast Livvy Dunne in the stands, Skenes threw a 99.8 mph fastball on the high, outside corner that Soto took for a called third strike. Judge struck out swinging at a sweeper that was outside.
A top contender for NL Rookie of the Year, the 22-year-old right-hander finished 11-3 with a 1.96 ERA in 23 starts and 133 innings — 29 innings shy of qualifying for the National League ERA title. He struck out 170 and walked 32.
“It was cool definitely glad to be able to finish the season,” Skenes said. “Cool to do it here, cool to do it against this lineup.”
Skenes is the first rookie pitcher with a sub-2.00 ERA and at least 23 starts in a season since Scott Perry for the 1918 Philadelphia Athletics, according to STATS,
“I wish I saw some more pitches,” Yankees catcher Austin Wells said. “He looked good out there and got six outs and that’s all I really saw.”
Gil allowed six runs and six hits in 5 2/3 innings, finishing with a 3.50 ERA. He had only allowed 14 homers in his first 28 starts.
“He’s had a great year,” Wells said. “Being a rookie starting pitcher for the Yankees and putting up what he did, he’s been amazing.”
Gonzales homered for the second straight game, a drive after his bat slipped away taking a practice swing in the on-deck circle. Yorke clanged his second career homer off the left-field foul pole.
“The guy that started for them is probably going to be the Rookie of the Year in the American League and is a special young arm, special pitcher and the fact that we had good at-bats and was able to grind, I was really proud of our group,” Shelton said.
Jazz Chisholm Jr. broke up Pittsburgh’s no-hit bid in the fifth with his 11th homer since being acquired from the Marlins on July 27 and then added an RBI double in the eighth. Soto hit an RBI single but ended the seventh by overrunning second base.
Mike Burrows (1-0), who underwent Tommy John surgery on his right elbow April 26, 2023, followed Skenes and threw 68 pitches over 3 1/3 innings to win in his major league debut. David Bednar struck out Chisholm to strand the bases loaded in the sixth.
Former Yankee Aroldis Chapman struck out Judge and retired Wells with the bases loaded for his 14th save in 19 chances.
TRAINER’S ROOM
Yankees: 1B Anthony Rizzo fractured the fourth and fifth fingers of his right hand when he was hit by RHP Ryan Borucki’s 88.2 mph slider in the seventh. Rizzo was replaced by INF Oswaldo Cabrera starting the eighth. Rizzo’s postseason status is uncertain.
UP NEXT
LHP Bailey Falter (8-9, 4.26) closes the regular season for the Pirates. RHP Clarke Schmidt (5-5, 2.55), a candidate for New York’s postseason rotation, makes his fifth start since returning a strained lat that sidelined him for more than three months.
AP MLB: https://apnews.com/hub/MLB
Pittsburgh Pirates' Aroldis Chapman pitches during the ninth inning of a baseball game against the New York Yankees, Saturday, Sept. 28, 2024, in New York. The Pirates won 9-4. (AP Photo/Frank Franklin II)
New York Yankees' Aaron Judge reacts after striking out during the sixth inning of a baseball game against the Pittsburgh Pirates, Saturday, Sept. 28, 2024, in New York. (AP Photo/Frank Franklin II)
New York Yankees catcher Austin Wells watches as Pittsburgh Pirates' Yasmani Grandal hits an RBI single during the ninth inning of a baseball game, Saturday, Sept. 28, 2024, in New York. (AP Photo/Frank Franklin II)
Pittsburgh Pirates' Nick Yorke (38) celebrates with Yasmani Grandal (6) after they scored on a two-run home run by Yorke during the ninth inning of a baseball game, Saturday, Sept. 28, 2024, in New York. (AP Photo/Frank Franklin II)
Pittsburgh Pirates' Paul Skenes smiles after the second inning of a baseball game against the New York Yankees, Saturday, Sept. 28, 2024, in New York. (AP Photo/Frank Franklin II)
Pittsburgh Pirates' Paul Skenes pitches during the first inning of a baseball game against the New York Yankees, Saturday, Sept. 28, 2024, in New York. (AP Photo/Frank Franklin II)
Pittsburgh Pirates' Paul Skenes pitches during the first inning of a baseball game against the New York Yankees, Saturday, Sept. 28, 2024, in New York. (AP Photo/Frank Franklin II)
